Tutorial: Getting Started

Thursday, 25th of January 2024 at 9:00 am – 10:45 am

Getting started all

Anika Rettig, Max-Planck-Institut für Molekulare Genetik, Berlin, Germany

Désirée Kunkel, BIH Cytometry Core Facility, Berlin Institute of Health (BIH) at Charite – Universitätsmedizin Berlin, Berlin, Germany

Axel R Schulz, Mass Cytometry Core Facility, German Rheumatism Research Center berlin, a Leibniz Institute, Berlin, Germany

Chair: Sarah Warth, Core Facility Cytometry, Ulm University, Ulm, Germany

Our introduction to mass cytometry ensures that everyone is at the same level when talking about this technology. Four experts from the field tell you how mass cytometry works and how it can be used to examine cell suspensions and tissue sections. We will guide you through typical experimental workflows and share our experience with important aspects in the application of mass cytometry, such as metal conjugation, sample barcoding, spillover compensation and batch normalization. You will also learn about the advantages of Imaging Mass Cytometry (IMC) and how to establish a multiplexed antibody panel for it. This is complemented by an introduction to current concepts of data analysis, both for imaging and suspension mass cytometry. Following the introductory talks there will also be time to discuss individual questions concerning mass cytometry and its application.