Monday, 26th of January at 9:00 am – 11:00 am
Désirée Kunkel, BIH Cytometry Core Facility, Berlin Institute of Health (BIH) at Charite – Universitätsmedizin Berlin, Berlin, Germany
Claudia Peitzsch, Mass Cytometry Facility, Center for Regenerative Therapies Dresden (CRTD), Dresden University of Technology, Dresden, Germany
Felix Röttele, Department of Medicine II, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
Axel R Schulz, Mass Cytometry Core Facility, German Rheumatism Research Center berlin, a Leibniz Institute, Berlin, Germany
Chair: Sarah Warth, Core Facility Cytometry, Ulm University, Ulm, Germany
Getting started – An Introduction to Mass Cytometry
This tutorial provides a comprehensive, practice-oriented introduction to mass cytometry for both suspension and imaging applications for beginners.
Our five experts will explain the principles of mass cytometry, demonstrate how it is applied to cell suspensions and tissue sections, and walk you through typical experimental workflows. You will gain hands-on insight into critical practical steps, including metal conjugation, sample barcoding, spillover correction, and strategies for minimizing batch effects and normalizing data across runs. A dedicated segment will highlight Imaging Mass Cytometry (IMC), outlining its key advantages and guiding you through the process of designing and establishing multiplexed antibody panels. This is complemented by an introduction to current concepts of data analysis, both for imaging and suspension mass cytometry. Following the introductory talks there will also be time to discuss individual questions concerning mass cytometry and its application.