Product Feature Talk by Ionpath

Friday, 26th of January 2024 at 2:05 pm

Chairs: Sabine Baumgart & Henrik Mei

Jay Tarolli

Jay Tarolli

Director of Product Development, Ionpath

Highly Multiplexed Spatial Proteomics with Interactive Insights Leading to Faster Answers

Multiplexed ion beam imaging (MIBI) has been developed to overcome many of the limitations of traditional proteomic imaging platforms for analyzing the tissue microenvironment, achieving true subcellular spatial resolution in combination with 40-plex labeling capacity in a single staining and acquisition. Staining tissue samples for MIBI imaging is similar to standard IHC protocols, allowing the technology to be easily adopted by users already in the spatial proteomics space. Based on time-of-flight secondary ion mass spectrometry, 40 metal tagged antibodies can be imaged simultaneously, providing a deep understanding of the tissue microenvironment. Ionpath recently launched MIBIplus to progress researchers from samples to answers in 6 weeks or less, enabling access to Ionpath’s expertise in sample preparation, imaging, and bioinformatics analysis. MIBIplus customers can send samples to Ionpath and receive a comprehensive report of the tissue microenvironment, including population statistics and distance metrics across 50+ cell types, as well as comparisons between sample groupings, such as responder and non-responder groups. Access to MIBIsight is included for MIBIplus customers which gives researchers access to interactive bioinformatics plots and control over the presented results without the need for access to a dedicated bioinformatics team.


Jay Tarolli earned a BS in Chemistry from Binghamton University in New York and a PhD in Analytical Chemistry from Penn State University. At Penn State, Jay studied in the lab of Nicholas Winograd where he improved the visual quality of time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of tissue and single cell biological images using a technique called image fusion. After graduating from Penn State, Jay completed a postdoctoral position at Pacific Northwest National Laboratory in Richland, WA where he continued working with SIMS imaging, furthering his image fusion work as well as developing a convolutional neural network for particle segmentation. In 2017, Jay joined Ionpath as a data scientist, applying his knowledge of ToF-SIMS data and image processing to the newly developed MIBI platform. During his career at Ionpath, Jay has also been Director of Applications where the team he led directly supported MIBI customers across the entire platform, from sample prep, image acquisition, and bioinformatics analysis. Currently, Jay is Director of Product Development, having overseen the launch of MIBIplus and the continued development of the entire MIBI platform.