Jena

Core Facility Cytometry

Medical Faculty

University Hospital Jena
Institute of Immunology
Leutragraben 3
07743 Jena, Germany

Prof. Diana Dudziak

Foto:Tino Zippel

Head

Prof. Diana Dudziak
Email: Diana.Dudziak@med.uni-jena.de

Phone: +49 3641 9 39500

Coordinator

Dr. Sabine Baumgart
Email: Sabine.Baumgart@med.uni-jena.de

Phone: +49 3641 9 397511

Webseite

Equipment
Helios (CyTOF instrument)

Established
2019

Service

  • Consulting
  • Panel design
  • Antibody conjugation (kits in storage)
  • Protocol optimization
  • Sample preparation
  • CyTOF measurement
  • Data pre-processing (normalization/debarcoding)
  • Data analysis

Keywords

Systems immunology
Oncoimmunology
Infection/Sepsis Immunology
Aging Reagents, barcoding
Human/mouse

Funding
Thuringian Ministry for Economic Affairs, Science and Digital Society

Publications

  1. Zhao L, Liu P, Mao M, Zhang S, Bigenwald C, Dutertre CA, Lehmann CHK, Pan H, Paulhan N, Amon L, Buque A, Yamazaki T, Galluzzi L, Kloeckner B, Silvin A, Pan Y, Chen H, Tian AL, Ly P, Dudziak D, Zitvogel L, Kepp O, Kroemer G. 2023. BCL2 inhibition reveals a dendritic cell-specific immune checkpoint that controls tumor immunosurveillance. Cancer Discov
  2. Heger L, Hatscher L, Liang C, Lehmann CHK, Amon L, Luhr JJ, Kaszubowski T, Nzirorera R, Schaft N, Dorrie J, Irrgang P, Tenbusch M, Kunz M, Socher E, Autenrieth SE, Purbojo A, Sirbu H, Hartmann A, Alexiou C, Cesnjevar R, Dudziak D. 2023. XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector functions from their immediate precursors. Proc Natl Acad Sci U S A 120: e2300343120
  3. Hatscher L, Kaszubowski T, Amon L, Dudziak D*, Heger L*. Circumventing pyroptosis via hyperactivation shapes superior immune responses of human type 2 dendritic cells compared to type 3 dendritic cells. Eur J Immunol. 2023 Sep;53(9):e2250123. doi: 10.1002/eji.202250123.
  4. Worm M, Glatzel V, Baumgart S, Mei HE, Sörensen T, Grützkau A, Heine G. Immune cell profiling reveals natural killer and T cell subpopulations to be associated with atopic dermatitis severity. Clin Exp Allergy. 2023 Jan;53(1):105-108. doi: 10.1111/cea.14228. Epub 2022 Oct 13. PMID: 36102492.
  5. Haunhorst S, Bloch W, Javelle F, Krüger K, Baumgart S, Drube S, Lemhöfer C, Reuken P, Stallmach A, Müller M, Zielinski CE, Pletz MW, Gabriel HHW, Puta C. A scoping review of regulatory T cell dynamics in convalescent COVID-19 patients – indications for their potential involvement in the development of Long COVID? Front Immunol. 2022 Dec 13;13:1070994. doi: 10.3389/fimmu.2022.1070994. PMID: 36582234; PMCID: PMC9792979.
  6. Hoang Y, Gryzik S, Hoppe I, Rybak A, Schädlich M, Kadner I, Walther D, Vera J, Radbruch A, Groth D, Baumgart S, Baumgrass R. PRI: Re-Analysis of a Public Mass Cytometry Dataset Reveals Patterns of Effective Tumor Treatments. Front Immunol. 2022 May 3;13:849329. doi: 10.3389/fimmu.2022.849329. PMID: 35592315; PMCID: PMC9110672.
  7. Amon L*, Dudziak D*, Backer RA, Clausen BE, Gmeiner C, Heger L, Jacobi L, Lehmann CHK, Probst HC, Seichter A, Tchitashvili G, Tochoedo NR, Trapaidze L, Vurnek D. 2022. Guidelines for DC preparation and flow cytometry analysis of mouse lymphohematopoietic tissues. Eur J Immunol n/a, *Lead author
  8. Clausen BE, Amon L, Backer RA, Berod L, Bopp T, Brand A, Burgdorf S, Chen L, Da M, Distler U, Dress RJ, Dudziak D, Dutertre CA, Eich C, Gabele A, Geiger M, Ginhoux F, Giusiano L, Godoy GJ, Hamouda AEI, Hatscher L, Heger L, Heidkamp GF, Hernandez LC, Jacobi L, Kaszubowski T, Kong WT, Lehmann CHK, Lopez-Lopez T, Mahnke K, Nitsche D, Renkawitz J, Reza RA, Saez PJ, Schlautmann L, Schmitt MT, Seichter A, Sielaff M, Sparwasser T, Stoitzner P, Tchitashvili G, Tenzer S, Tochoedo NR, Vurnek D, Zink F, Hieronymus T. 2022. Guidelines for mouse and human DC functional assays. Eur J Immunol n/a
  9. Heger L*, Dudziak D*, Amon L, Hatscher L, Kaszubowski T, Lehmann CHK. 2022. Guidelines for DC preparation and flow cytometric analysis of human lymphohematopoietic tissues. Eur J Immunol n/a: e2249917, *Lead author
  10. Probst HC, Stoitzner P, Amon L, Backer RA, Brand A, Chen J, Clausen BE, Dieckmann S, Dudziak D, Heger L, Hodapp K, Hornsteiner F, Hovav AH, Jacobi L, Ji X, Kamenjarin N, Lahl K, Lahmar I, Lakus J, Lehmann CHK, Ortner D, Picard M, Roberti MP, Rossnagel L, Saba Y, Schalla C, Schlitzer A, Schraml BU, Schutze K, Seichter A, Sere K, Seretis A, Sopper S, Strandt H, Sykora MM, Theobald H, Tripp CH, Zitvogel L. 2022. Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues. Eur J Immunol n/a
  11. Lutz MB, Ali S, Audiger C, Autenrieth SE, Berod L, Bigley V, Cyran L, Dalod M, Dörrie J, Dudziak D, Flórez-Grau G, Giusiano L, Godoy GJ, Heuer M, Krug AB, Lehmann CHK, Mayer CT, Naik SH, Scheu S, Schreibelt G, Segura E, Seré K, Sparwasser T, Tel J, Xu H, Zenke M. Guidelines for mouse and human DC generation. Eur J Immunol. 2022 Oct 27. doi: 10.1002/eji.202249816. PMID: 36303448
  12. Dudziak D*, Heger L*, Agace WW, Bakker J, de Gruijl TD, Dutertre C-A, Dress RJ, Fenton TM, Fransen MF, Ginhoux F, Heyman O, Horev Y, Hornsteiner F, Yona S, Kandiah V, Kles P, Lubin R, Mizraji G, Prokopi A, Saar O, Sopper S, Stoitzner P, Strandt H, Sykora MM, Toffoli E, Tripp CH, van Pul K, van de Ven R, Wilensky A, Zelle-Rieser C. Guidelines on preparation and flow cytometric analysis of human non-lymphoid tissue DC. Eur J Immunol. In Revision. *Lead author
  13. Hatscher L, Lehmann CHK, Purbojo A, Onderka C, Liang C, Hartmann A, Cesnjevar R, Bruns H, Gross O, Nimmerjahn F, Ivanović-Burmazović I, Kunz M, Heger L, Dudziak D. (2021). Select hyperactivating NLRP3 ligands enhance the TH1- and TH17-inducing potential of human type 2 conventional dendritic cells. Sci Signal. 14(680):eabe1757. doi: 10.1126/scisignal.abe1757
  14. Günther A, Brämer D, Pletz MW, Kamradt T, Baumgart S, Mayer TE, Baier M, Autsch A, Mawrin C, Schönborn L, Greinacher A, Thiele T. Complicated Long Term Vaccine Induced Thrombotic Immune Thrombocytopenia-A Case Report. Vaccines (Basel). 2021 Nov 17;9(11):1344. doi: 10.3390/vaccines9111344. PMID: 34835275; PMCID: PMC8622649.
  15. Reuken PA, Stallmach A, Pletz MW, Brandt C, Andreas N, Hahnfeld S, Löffler B, Baumgart S, Kamradt T, Bauer M. Severe clinical relapse in an immunocompromised host with persistent SARS-CoV-2 infection. Leukemia. 2021 Mar;35(3):920-923. doi: 10.1038/s41375-021-01175-8. Epub 2021 Feb 19. PMID: 33608636; PMCID: PMC7893131.
  16. Papaioannou NE, Salei N, Rambichler S, Ravi K, Popovic J, Küntzel V, Lehmann CHK, Fian­ce­t­te R, Salvermoser J, Gajdasik DW, Mettler R, Messerer D, Carrelha J, Ohnmacht C, Hal­ler D, Stumm R, Straub T, Jacobsen SEW, Schulz C, Withers DR, Schotta G, Dudziak D, Sch­r­a­ml BU. (2021). Environmental signals rather than layered ontogeny imprint the function of type 2 conventional dendritic cells in young and adult mice. Nat Commun. 12(1):464. doi: 10.1038/s41467-020-20659-2.
  17. Szumilas N, Corneth OBJ, Lehmann CHK, Schmitt H, Cunz S, Cullen JG, Chu T, Marosan A, Mócsai A, Benes V, Zehn D, Dudziak D, Hendriks RW, Nitschke L. Siglec-H-Deficient Mice Show Enhanced Type I IFN Responses, but Do Not Develop Autoimmunity After Influenza or LCMV Infections. Front Immunol. 2021 Aug 23;12:698420. doi: 10.3389/fimmu.2021.698420. eCollection 2021. PMID: 34497606
  18. Bertolo M*, Baumgart S*, Durek P, Peddinghaus A, Mei H, Rose T, Enghard P, Grützkau A. Deep Phenotyping of Urinary Leukocytes by Mass Cytometry Reveals a Leukocyte Signature for Early and Non-Invasive Prediction of Response to Treatment in Active Lupus Nephritis. Front Immunol. 2020 Mar 24;11:256. doi: 10.3389/fimmu.2020.00256. PMID: 32265898; PMCID: PMC7105605.
    *Shared first authors
  19. Buchele V, Konein P, Vogler T, Kunert T, Enderle K, Khan H, Büttner-Herold M, Lehmann CHK, Amon L, Wirtz S, Dudziak D, Neurath MF, Neufert C, Hildner K. Th17 Cell-Mediated Colitis Is Positively Regulated by Interferon Regulatory Factor 4 in a T Cell-Extrinsic Manner. Front Immunol. 2021 Jan 29;11:590893. doi: 10.3389/fimmu.2020.590893. eCollection 2020. PMID: 33584655
  20. Lühr JJ, Alex N, Amon L, Kräter M, Kubánková M, Sezgin E, Lehmann CHK, Heger L, Heidkamp GF, Smith AS, Zaburdaev V, Böckmann RA, Levental I, Dustin ML, Eggeling C, Guck J, Dudziak D. Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition. Front Immunol. 2020 Nov 27;11:590121. doi: 10.3389/fimmu.2020.590121. eCollection 2020. PMID: 33329576
  21. Picard M, Yonekura S, Slowicka K, Petta I, Rauber C, Routy B, Richard C, Iebba V, Tidjani Alou M, Becharef S, Ly P, Pizzato E, Lehmann CHK, Amon L, Klein C, Opolon P, Gomperts Boneca I, Scoazec JY, Hollebecque A, Malka D, Ghiringhelli F, Dudziak D, Berx G, Vereecke L, van Loo G, Kroemer G, Zitvogel L, Roberti MP. Ileal immune tonus is a prognosis marker of proximal colon cancer in mice and patients. Cell Death Differ. 2021 May;28(5):1532-1547. doi: 10.1038/s41418-020-00684-w. Epub 2020 Dec 1. PMID: 33262469
  22. Danzer H, Glaesner J, Baerenwaldt A, Reitinger C, Lux A, Heger L, Dudziak D, Harrer T, Gessner A, Nimmerjahn F. Human Fcγ-receptor IIb modulates pathogen-specific versus self-reactive antibody responses in lyme arthritis. Elife. 2020 Jul 2;9:e55319. doi: 10.7554/eLife.55319. PMID: 32613944
  23. Roberti MP, Yonekura S, Duong CPM, Picard M, Ferrere G, Tidjani Alou M, Rauber C, Iebba V, Lehmann CHK, Amon L, Dudziak D, Derosa L, Routy B, Flament C, Richard C, Daillère R, Fluckiger A, Van Seuningen I, Chamaillard M, Vincent A, Kourula S, Opolon P, Ly P, Pizzato E, Becharef S, Paillet J, Klein C, Marliot F, Pietrantonio F, Benoist S, Scoazec JY, Dartigues P, Hollebecque A, Malka D, Pagès F, Galon J, Gomperts Boneca I, Lepage P, Ryffel B, Raoult D, Eggermont A, Vanden Berghe T, Ghiringhelli F, Vandenabeele P, Kroemer G, Zitvogel L. (2020). Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer. Nat Med. 26(6):919-931. doi: 10.1038/s41591-020-0882-8.
  24. 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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol. 2019 Oct;49(10):1457-1973. doi: 10.1002/eji.201970107. PMID: 31633216
  25. Dudeck J, Froebel J, Kotrba J, Lehmann CHK, Dudziak D, Speier S, Nedospasov SA, Schraven B, Dudeck A. Engulfment of mast cell secretory granules on skin inflammation boosts dendritic cell migration and priming efficiency. J Allergy Clin Immunol. 2019 May;143(5):1849-1864.e4. doi: 10.1016/j.jaci.2018.08.052. Epub 2018 Oct 17. PMID: 30339853
  26. Schulz AR, Baumgart S, Schulze J, Urbicht M, Grützkau A, Mei HE. Stabilizing Antibody Cocktails for Mass Cytometry. Cytometry A. 2019 Aug;95(8):910-916. doi: 10.1002/cyto.a.23781. Epub 2019 May 6. PMID: 31058420.
  27. Budzinski L, Schulz AR, Baumgart S, Burns T, Rose T, Hirseland H, Mei HE. Osmium-Labeled Microspheres for Bead-Based Assays in Mass Cytometry. J Immunol. 2019 May 15;202(10):3103-3112. doi: 10.4049/jimmunol.1801640. Epub 2019 Apr 15. PMID: 30988119.
  28. Guo Y, Baumgart S, Stärk HJ, Harms H, Müller S. Mass Cytometry for Detection of Silver at the Bacterial Single Cell Level. Front Microbiol. 2017 Jul 17;8:1326. doi: 10.3389/fmicb.2017.01326. PMID: 28769897; PMCID: PMC5511850.
  29. Baumgart S, Schulz AR, Peddinghaus A, Stanislawiak S, Gillert S, Hirseland H, Krauthäuser S, Dose C, Mei HE, Grützkau A. Dual-labelled antibodies for flow and mass cytometry: A new tool for cross-platform comparison and enrichment of target cells for mass cytometry. Eur J Immunol. 2017 Aug;47(8):1377-1385. doi: 10.1002/eji.201747031. Epub 2017 Jul 18. PMID: 28654217.
  30. Baumgart S, Peddinghaus A, Schulte-Wrede U, Mei HE, Grützkau A. OMIP-034: Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies. Cytometry A. 2017 Jan;91(1):34-38. doi: 10.1002/cyto.a.22894. Epub 2016 Jun 30. PMID: 27362704.
  31. Schulz AR, Stanislawiak S, Baumgart S, Grützkau A, Mei HE. Silver nanoparticles for the detection of cell surface antigens in mass cytometry. Cytometry A. 2017 Jan;91(1):25-33. doi: 10.1002/cyto.a.22904. Epub 2016 Jun 28. PMID: 27351740.
  32. Lehmann B, Biburger M, Brückner C, Ipsen-Escobedo A, Gordan S, Lehmann C, Voehringer D, Winkler T, Schaft N, Dudziak D, Sirbu H, Weber GF, Nimmerjahn F. Tumor location determines tissue-specific recruitment of tumor-associated macrophages and antibody-dependent immunotherapy response.Sci Immunol. 2017 Jan 6;2(7):eaah6413. doi: 10.1126/sciimmunol.aah6413. PMID: 28783667
  33. Lehmann CHK, Baranska A, Heidkamp GF, Heger L, Neubert K, Lühr JJ, Hoffmann A, Reimer KC, Brückner C, Beck S, Seeling M, Kießling M, Soulat D, Krug AB, Ravetch JV, Leusen JHW, Nimmerjahn F*, Dudziak D*. (2017). DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo. J Exp Med. 214(5):1509-1528. doi: 10.1084/jem.20160951.
  34. Pfeifle R, Rothe T, Ipseiz N, Scherer HU, Culemann S, Harre U, Ackermann JA, Seefried M, Kleyer A, Uderhardt S, Haugg B, Hueber AJ, Daum P, Heidkamp GF, Ge C, Böhm S, Lux A, Schuh W, Magorivska I, Nandakumar KS, Lönnblom E, Becker C, Dudziak D, Wuhrer M, Rombouts Y, Koeleman CA, Toes R, Winkler TH, Holmdahl R, Herrmann M, Blüml S, Nimmerjahn F, Schett G, Krönke G. Regulation of autoantibody activity by the IL-23-TH17 axis determines the onset of autoimmune disease. Nat Immunol. 2017 Jan;18(1):104-113. doi: 10.1038/ni.3579. Epub 2016 Nov 7. PMID: 27820809
  35. Heidkamp GF*, Sander J*, Lehmann CHK, Heger L, Eissing N, Baranska A, Lühr JJ, Hoffmann A, Reimer KC, Lux A, Söder S, Hartmann A, Zenk J, Ulas T, McGovern N, Alexiou C, Spriewald B, Mackensen A, Schuler G, Schauf B, Forster A, Repp R, Fasching PA, Purbojo A, Cesnjevar R, Ullrich E, Ginhoux F, Schlitzer A, Nimmerjahn F, Schultze JL*, Dudziak D *. (2016). Human lymphoid organ dendritic cell identity is predominantly dictated by ontogeny, not tissue microenvironment. Sci Immunol. 1(6):eaai7677. doi: 10.1126/sciimmunol.aai.7677. First total organ analysis of human tissues for DCs.
  36. Gerstner S, Köhler W, Heidkamp G, Purbojo A, Uchida S, Ekici AB, Heger L, Luetke-Eversloh M, Schubert R, Bader P, Klingebiel T, Koehl U, Mackensen A, Romagnani C, Cesnjevar R, Dudziak D, Ullrich E. Specific phenotype and function of CD56-expressing innate immune cell subsets in human thymus. J Leukoc Biol. 2016 Dec;100(6):1297-1310. doi: 10.1189/jlb.1A0116-038R. Epub 2016 Jun 28. PMID: 27354408
  37. Schleicher U, Paduch K, Debus A, Obermeyer S, König T, Kling JC, Ribechini E, Dudziak D, Mougiakakos D, Murray PJ, Ostuni R, Körner H, Bogdan C. TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection. Cell Rep. 2016 May 3;15(5):1062-75. doi: 10.1016/j.celrep.2016.04.001. Epub 2016 Apr 21. PMID: 27117406
  38. Dudeck J, Ghouse SM, Lehmann CH, Hoppe A, Schubert N, Nedospasov SA Dudziak D, Dudeck A. Mast-Cell-Derived TNF Amplifies CD8(+) Dendritic Cell Functionality and CD8(+) T Cell Priming. Cell Rep. 2015 Oct 13;13(2):399-411. doi: 10.1016/j.celrep.2015.08.078. Epub 2015 Sep 24. PMID: 26411682
  39. Sörensen T, Baumgart S, Durek P, Grützkau A, Häupl T. immunoClust–An automated analysis pipeline for the identification of immunophenotypic signatures in high-dimensional cytometric datasets. Cytometry A. 2015 Jul;87(7):603-15. doi: 10.1002/cyto.a.22626. Epub 2015 Apr 7. PMID: 25850678.
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