The mass cytometry facility at CRTD in Dresden
Contact:
Dr. Claudia Peitzsch
Center for Regenerative Therapies Dresden (CRTD)
Center for Molecular and Cellular Bioengineering (CMCB)
Medical Faculty Carl Gustav Carus
Dresden University of Technology (TU Dresden)
Fetcherstr. 105, 01307 Dresden, Germany
Tel: +49 351 458-82176
Email: claudia.peitzsch@tu-dresden.de
© Magdalena-Gonciarz
© Sevina Dietz
Sevina Dietz
Mass cytometry specialist
Research group of Prof. Ezio Bonifacio
Center for Regenerative Therapies Dresden (CRTD)
Medical Faculty Carl Gustav Carus, Dresden University of Technology
Fetcherstr. 105, 01307 Dresden, Germany
Tel: +49 351 458-82149
Email: sevina.dietz@tu-dresden.de
Offered services at the mass cytometry facility:
- Assistance for experimental planning, including panel design and controls
- Training for sample processing and staining
- Costum-made antibody-metal conjugation
- Available panel for deep immune cell profiling, intracellular cytokine expression and tyrosine kinase phosphorylation
- Service for suspension mass cytometry measurements
- Access to data analysis tools
- Webpage: https://tu-dresden.de/cmcb/technologie-plattform/facilities/mass-cytometry
Main topics of interest
- Deep immune cell profiling of human whole blood and isolated PBMC samples
- Standardization for clinical setup e.g., for patient samples with infectious disease, autoimmune disorders, cancer or after cell-based therapies
- Application of mass cytometry for functional analysis including intracellular cytokines, tyrosine kinase phosphorylation, histone modification and metabolic enzymes
- Phenotyping of human organoids, solid tumors or murine samples
Instrumentation
- CyTOF2, Fluidigm (since 2014)
- CyTOF XT, Standard BioTools (since 2023)
© Sevina Dietz
Publications
Singh et al. 2018, Stem Cell Reports: Hematopoietic Stem Cells but Not Multipotent Progenitors Drive Erythropoiesis during Chronic Erythroid Stress in EPO Transgenic Mice
https://www.sciencedirect.com/science/article/pii/S2213671118301802?via%3Dihub
Ramasz et al. 2019, Blood: Hematopoietic stem cell response to acute thrombocytopenia requires signaling through distinct receptor tyrosine kinases
https://doi.org/10.1182/blood.2019000721